Anti-Human CD25/IL2R alpha Recombinant Antibody (1H4), APC (HF996137)

Name: Anti-Human CD25/IL2R alpha Recombinant Antibody (1H4), APC
Brand: abinScience
SKU: HF996137
Price: 256 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HF996137
Species reactivity	Human
Applications	FCM
Host species	Human
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone ID	1H4
Conjugation	APC
Tested applications	FCM
Target	CD25, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, TAC antigen, p55
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Associated products	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
Accession	P01589
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Interleukin-2 receptor subunit alpha (CD25/IL2RA) is a ~30 kDa protein. Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow cytometry

Flow-cytometry APC using anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD25 monoclonal antibody (Catalog HF996137) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet