Anti-Human CD27/TNFRSF7 Recombinant Antibody (1F5), PerCP (HB796347)

Name: Anti-Human CD27/TNFRSF7 Recombinant Antibody (1F5), PerCP
Brand: abinScience
SKU: HB796347
Price: 268 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50T
100T

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HB796347
Description	Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F5), PerCP (HB796347) is a human monoclonal antibody detecting CD27 Ligand/TNFSF7/CD70 in FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●PerCP Conjugated — Ready for direct detection without secondary antibody.
Species reactivity	Human
Applications	FCM
Host species	Human
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone ID	1F5
Conjugation	PerCP
Target	CD27/TNFRSF7
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Associated products	Human IgG1 kappa Recombinant Isotype Control Antibody (HyHEL-10), PerCP
Purification	Affinity chromatography.
Accession	P26842
Form	Liquid
Storage buffer	0.01M PBS pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	CD27 antigen is a ~29 kDa protein. Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. 1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863 2. Jaeger-Ruckstuhl, CA. et al. (2024) Immunity 57, 287-302.e12. PMID: 38354704
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

References

Datasheet

Anti-Human CD27/TNFRSF7 Recombinant Antibody (1F5), PerCP.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CD27/TNFRSF7 Recombinant Antibody (1F5), PerCP (HB796347)

Overview

Images

References

Datasheet

SDS