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Anti-Human CD272/BTLA Recombinant Antibody (SAA0087), FITC (HV375117)

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Anti-Human CD272/BTLA Recombinant Antibody (SAA0087), FITC

Anti-Human CD272/BTLA Recombinant Antibody (SAA0087), FITC

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Overview

Catalog No.	HV375117
Description	Anti-Human CD272/BTLA Antibody (SAA0087), FITC [SAA0087] (HV375117) is a human monoclonal antibody detecting CD272 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG4, kappa
Clone ID	SAA0087
Conjugation	FITC
Clonality	Monoclonal
Target	B- and T-lymphocyte attenuator, BTLA, CD272, B- and T-lymphocyte-associated protein
Endotoxin level	Please contact with the lab for this information.
Accession	Q7Z6A9
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	B- and T-lymphocyte attenuator (CD272/BTLA) is a ~32 kDa protein. Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells. 1. Watanabe, N. et al. (2003) Nature immunology 4, 670-9. PMID: 12796776 2. Gavrieli, M. et al. (2003) Biochemical and biophysical research communications 312, 1236-43. PMID: 14652006 3. Sedy, JR. et al. (2005) Nature immunology 6, 90-8. PMID: 15568026 4. Cai, G. et al. (2008) Nature immunology 9, 176-85. PMID: 18193050 5. Cheung, TC. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 183, 7286-96. PMID: 19915044
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow cytometry

Flow-cytometry FITC using anti-human CD272 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human CD272 monoclonal antibody (Catalog HV375117) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD272/BTLA Recombinant Antibody (SAA0087), FITC.pdf

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Anti-Human CD272/BTLA Recombinant Antibody (SAA0087), FITC