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Anti-Human CD275/ICOSLG Recombinant Antibody (SAA0089), PE (HF564127)

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Anti-Human CD275/ICOSLG Recombinant Antibody (SAA0089), PE

Anti-Human CD275/ICOSLG Recombinant Antibody (SAA0089), PE

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Overview

Catalog No.	HF564127
Description	Anti-Human CD275/ICOSLG Antibody (SAA0089), PE [SAA0089] (HF564127) is a human monoclonal antibody detecting ICOSLG in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG2, kappa
Clone ID	SAA0089
Conjugation	PE
Clonality	Monoclonal
Target	B7RP1, B7-related protein 1, CD275, ICOSL, ICOSLG, KIAA0653, B7 homolog 2, ICOS ligand, B7RP-1, B7H2, B7-H2, B7-like protein Gl50
Endotoxin level	Please contact with the lab for this information.
Accession	O75144
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	ICOS ligand (CD275/ICOSLG) is a ~33 kDa protein. Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion. Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP). 1. Yoshinaga, SK. et al. (2000) International immunology 12, 1439-47. PMID: 11007762 2. Wang, S. et al. (2000) Blood 96, 2808-13. PMID: 11023515 3. Roussel, L. et al. (2018) The Journal of experimental medicine 215, 3151-3164. PMID: 30498080
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using PE anti-human CD275 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD275 monoclonal antibody (Catalog: HF564127, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD275/ICOSLG Recombinant Antibody (SAA0089), PE.pdf

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Anti-Human CD275/ICOSLG Recombinant Antibody (SAA0089), PE