Anti-Human CD276/B7-H3 Recombinant Antibody (SAA2010) (HV612013)

Name: Anti-Human CD276/B7-H3 Recombinant Antibody (SAA2010)
Brand: abinScience
SKU: HV612013
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV612013

Description

Anti-Human CD276/B7-H3 Recombinant Antibody (SAA2010) (HV612013) is a human monoclonal antibody detecting CD276/B7-H3 in ELISA, FCM, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, FCM, WB

Host species

Human

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone ID

SAA2010

Tested applications

FCM

Target

4Ig-B7-H3, B7 homolog 3, B7-H3, B7H3, CD276, CD276 antigen, Costimulatory molecule

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q5ZPR3

RRID

Anti-Human CD276/B7-H3 Antibody (SAA2010) (abinScience Cat# HV612013, RRID:AB_3727922)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

CD276 antigen (B7-H3) is a ~57 kDa protein. May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. CD276 is the therapeutic target of omburtamab (clinical).

Note

For research use only.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Images

Flow Cytometry

Flow-cytometry using anti-human CD276 antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD276 antibody monoclonal antibody (Catalog # HV612013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD276/B7-H3 Antibody (SAA2010).

References

Datasheet

Anti-Human CD276/B7-H3 Recombinant Antibody (SAA2010).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CD276/B7-H3 Recombinant Antibody (SAA2010) (HV612013)

Overview

Images

References

Datasheet

SDS