Anti-Human CD276/B7-H3 Antibody (SAA2010) (HV612013)

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Overview
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Datasheet

Overview

Catalog No.

HV612013

Description

Anti-Human CD276/B7-H3 Antibody (SAA2010) [SAA2010] (HV612013) is a human monoclonal antibody detecting B7-H3 in ELISA, FCM, WB. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

ELISA, FCM, WB

Host species

Human

Isotype

IgG1, kappa

Clone ID

SAA2010

Clonality

Monoclonal

Target

CD276, B7-H3, Costimulatory molecule, B7H3, 4Ig-B7-H3, B7 homolog 3, CD276 antigen

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q5ZPR3

RRID

Anti-Human CD276/B7-H3 Antibody (SAA2010) (abinScience Cat# HV612013, RRID:AB_3727922)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

CD276 antigen (B7-H3) is a ~57 kDa protein. May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. CD276 is the therapeutic target of omburtamab (clinical).

Note

For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human CD276 antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD276 antibody monoclonal antibody (Catalog # HV612013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD276/B7-H3 Antibody (SAA2010).