Home > Products > Antibodies > Flow Cytometry (FACS) Antibodies

Anti-Human CD278/ICOS Recombinant Antibody (SAA0091), FITC (HV702117)

Datasheet Share product Copy info

Anti-Human CD278/ICOS Recombinant Antibody (SAA0091), FITC

Anti-Human CD278/ICOS Recombinant Antibody (SAA0091), FITC

Price(USD): $

Spec:

50T
100T

Number:

Contact us

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HV702117
Description	Anti-Human CD278/ICOS Antibody (SAA0091), FITC [SAA0091] (HV702117) is a human monoclonal antibody detecting CD278 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	SAA0091
Conjugation	FITC
Clonality	Monoclonal
Target	CD278, ICOS, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, AILIM
Endotoxin level	Please contact with the lab for this information.
Accession	Q9Y6W8
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Inducible T-cell costimulator (CD278/ICOS) is a ~22 kDa protein. Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response. Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection. Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling. In addition, activates PI3K signaling pathways independently of calcium flux. 1. Hutloff, A. et al. (1999) Nature 397, 263-6. PMID: 9930702 2. Rujas, E. et al. (2020) Nature communications 11, 5066. PMID: 33033255 3. Pedros, C. et al. (2016) Nature immunology 17, 825-33. PMID: 27135603 4. Wan, Z. et al. (2020) Cellular & molecular immunology 17, 143-152. PMID: 30523347
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using FITC anti-human CD278 antibody. MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD278 monoclonal antibody (Catalog: HV702117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD278/ICOS Recombinant Antibody (SAA0091), FITC.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

=

Concentration

×

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

×

V₁ (Stock Vol.)

=

Working Solution

C₂ (Working Conc.)

×

V₂ (Working Vol.)

Recommendation

Contact us for custom quotes, bulk requests and any other issues.

Mail: support@abinScience.com

Distributor list

Anti-Human CD278/ICOS Recombinant Antibody (SAA0091), FITC