Anti-Human CD28 Recombinant Antibody (TGN1412) (HY422023)

Name: Anti-Human CD28 Recombinant Antibody (TGN1412)
Brand: abinScience
SKU: HY422023
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY422023

Species reactivity

Human

Applications

ELISA, WB, FCM, Stimulation

Host species

Human

Isotype

IgG4, kappa

Clone ID

TGN1412

Expression system

Mammalian cells

Clonality

Monoclonal

Target

CD28

Endotoxin level

Please contact with the lab for this information.

Purity

>95% purity as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P10747

RRID

Anti-Human CD28 Antibody (TGN1412) (abinScience Cat# HY422023, RRID:AB_3729830)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.

Alternate Names

CD28 superagonist,CD28SA, TAB08

Background

T-cell-specific surface glycoprotein CD28 is a ~25 kDa protein. Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis. Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.

1. Gimmi, CD. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 6575-9. PMID: 1650475

2. Raab, M. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 8891-5. PMID: 7568038

3. Skånland, SS. et al. (2014) The Biochemical journal 460, 399-410. PMID: 24665965

4. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291

5. Wu, H. et al. (2022) Structure (London, England : 1993) 30, 803-812.e5. PMID: 35397202

6. Kong, KF. et al. (2011) Nature immunology 12, 1105-12. PMID: 21964608

7. Blotta, MH. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3133-40. PMID: 8617933

Note

For research use only.

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