Home > Products > Antibodies > Flow Cytometry (FACS) Antibodies

Anti-Human CD309/KDR/VEGFR-2 Recombinant Antibody (1121B), FITC (HW338117)

Datasheet Share product Copy info

Anti-Human CD309/KDR/VEGFR-2 Recombinant Antibody (1121B), FITC

Anti-Human CD309/KDR/VEGFR-2 Recombinant Antibody (1121B), FITC

Price(USD): $

Spec:

50T
100T

Number:

Contact us

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HW338117
Description	Anti-Human CD309/KDR/VEGFR-2 Antibody (1121B), FITC [1121B] (HW338117) is a mouse monoclonal antibody detecting VEGFR-2 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	1121B
Conjugation	FITC
Clonality	Monoclonal
Target	FLK-1, Kinase insert domain receptor, VEGFR2, Fetal liver kinase 1, CD309, Vascular endothelial growth factor receptor 2, Protein-tyrosine kinase receptor flk-1, FLK1, VEGFR-2, KDR
Endotoxin level	Please contact with the lab for this information.
Accession	P35968
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Vascular endothelial growth factor receptor 2 (CD309/VEGFR-2) is a ~151 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. CD309 is the therapeutic target of ramucirumab (Cyramza).
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using FITC anti-human CD309 antibody. HUVEC cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD309 monoclonal antibody (Catalog: HW338117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD309/KDR/VEGFR-2 Recombinant Antibody (1121B), FITC.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

=

Concentration

×

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

×

V₁ (Stock Vol.)

=

Working Solution

C₂ (Working Conc.)

×

V₂ (Working Vol.)

Recommendation

Contact us for custom quotes, bulk requests and any other issues.

Mail: support@abinScience.com

Distributor list

Anti-Human CD309/KDR/VEGFR-2 Recombinant Antibody (1121B), FITC