

| Catalog No. | HV451137 |
|---|---|
| Species reactivity | Human |
| Applications | FCM |
| Host species | Mouse |
| Isotype | IgG1, kappa |
| Clone ID | CUB4 |
| Conjugation | APC |
| Clonality | Monoclonal |
| Target | CD318, Transmembrane and associated with src kinases, TRASK, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Membrane glycoprotein gp140, CUB domain-containing protein 1, CDCP1 |
| Endotoxin level | Please contact with the lab for this information. |
| Associated products | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC |
| Accession | Q9H5V8 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Background | CUB domain-containing protein 1 (CD318/CDCP1) is a ~92 kDa protein. May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. |
| Note | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |

Flow-cytometry using APC anti-human CD318 antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD318 monoclonal antibody (Catalog: HV451137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.




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