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Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3) (HY139107)

Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3)
Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3)
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Overview
Catalog No.HY139107
Description
Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3) (HY139107) is a human monoclonal antibody detecting Fc gamma RIIA/CD32a in ELISA, FCM, IF, IP. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, FCM, IF, IP
Host speciesHuman
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone IDIV.3
Tested applications FCM
Target Fc-gamma RII-a, IgG Fc receptor II-a, IGFR2, FCG2, CDw32, FCGR2A1, FCGR2A, FcRII-a, CD32, Low affinity immunoglobulin gamma Fc region receptor II-a, Fc-gamma-RIIa
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P12318
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Low affinity immunoglobulin gamma Fc region receptor II-a (CD32/FCGR2A) is a ~35 kDa protein. Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.

Note For research use only.
Images
  • Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3)

    Flow Cytometry

    Flow-cytometry using anti-human CD32 antibody.CD32 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD32 antibody monoclonal antibody (Catalog # HY139107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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