Anti-Human MICL/CLEC12A Recombinant Antibody (SAA0102), APC (HV493137)

Name: Anti-Human MICL/CLEC12A Recombinant Antibody (SAA0102), APC
Brand: abinScience
SKU: HV493137
Price: 290 USD
Availability: InStock

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Overview

Catalog No.	HV493137
Description	Anti-Human MICL/CLEC12A Recombinant Antibody (SAA0102), APC (HV493137) is a mouse monoclonal antibody detecting MICL/CLEC12A in FCM. Suitable for Human. Highlights ●APC Conjugated — Ready for direct detection without secondary antibody.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Clonality	Monoclonal
Isotype	IgG2a, kappa
Clone ID	SAA0102
Conjugation	APC
Target	CLEC12A, DCAL2, Dendritic cell-associated lectin 2, CLL-1, Myeloid inhibitory C-type lectin-like receptor, C-type lectin domain family 12 member A, CLL1, DCAL-2, C-type lectin-like molecule 1, MICL, CD371
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Associated products	Mouse IgG2a kappa Recombinant Isotype Control Antibody (MOPC-173), APC
Accession	Q5QGZ9
Form	Liquid
Storage buffer	0.01M PBS pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	C-type lectin domain family 12 member A (CD371/CLEC12A) is a ~30 kDa protein. Myeloid inhibitory C-type lectin receptor that acts as a negative regulator of myeloid cell activation. Myeloid cell inhibition is required to limit proinflammatory pathways and protect against excessive inflammation. Specifically recognizes and binds various structures, such as neutrophil extracellular traps (NETs) or monosodium urate crystals. Also acts as a pattern-recognition receptor for pathogen-associated molecules, such as plasmodium hemozoin or mycobacterial micolic acid. Ligand-binding induces phosphorylation of its ITIM motif, followed by recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11, which counteract tyrosine-protein kinase SYK, thereby preventing myeloid cell activation. 1. Marshall, AS. et al. (2004) The Journal of biological chemistry 279, 14792-802. PMID: 14739280 2. Han, Y. et al. (2004) Blood 104, 2858-66. PMID: 15238421 3. Chen, CH. et al. (2006) Blood 107, 1459-67. PMID: 16239426 4. Paré, G. et al. (2021) Frontiers in immunology 12, 650808. PMID: 34234773 5. Tang, H. et al. (2024) The Journal of biological chemistry 300, 105765. PMID: 38367667 6. Mori, S. et al. (2024) International immunology 36, 279-290. PMID: 38386511 7. Malamud, M. et al. (2024) Nature 633, 442-450. PMID: 39143217
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow cytometry

Flow-cytometry APC using anti-human CD371 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD371 monoclonal antibody (Catalog HV493137) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human MICL/CLEC12A Recombinant Antibody (SAA0102), APC.pdf

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Anti-Human MICL/CLEC12A Recombinant Antibody (SAA0102), APC (HV493137)

Overview

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References

Datasheet

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