Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242) (HY057043)

Name: Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242)
Brand: abinScience
SKU: HY057043
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY057043

Description

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Anti-Human CD3E Nanobody (SAA2242) [SAA2242] (HY057043) is a alpaca monoclonal antibody detecting CD3e in ELISA, FCM. Suitable for Human.

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Highlights

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●Flow Cytometry — Validated for flow cytometry applications.

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Species reactivity

Human

Applications

ELISA, FCM

Host species

Alpaca

Isotype

VHH-His

Clone ID

SAA2242

Clonality

Monoclonal

Target

T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P07766

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto).

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1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938

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2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002

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3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

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4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325

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5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660

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Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human CD3E Nanobody (SAA2242)
Bioactivity

Detects recombinant human CD3E (Catalog No: HY057031) in indirect ELISA.
Flow cytometry

Flow cytometry analysis was performed using anti-human CD3 monoclonal antibody. Jurkat cells were incubated with an irrelevant antibody (blue histogram) or FITC-conjugated anti-human CD3 monoclonal antibody (Catalog # HY057043, green histogram) at a concentration of 5 µg/mL for 30 min at room temperature. After washing, cells were directly analyzed on a NovoCyte Flow Cytometer.