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Anti-Human CD4 Recombinant Antibody (5A8) (HF998207)

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Anti-Human CD4 Recombinant Antibody (5A8)

Anti-Human CD4 Recombinant Antibody (5A8)

Anti-Human CD4 Recombinant Antibody (5A8)

Anti-Human CD4 Recombinant Antibody (5A8)

Anti-Human CD4 Recombinant Antibody (5A8)

Anti-Human CD4 Recombinant Antibody (5A8)

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HF998207
Description	Anti-Human CD4 Antibody (5A8) [5A8] (HF998207) is a mouse monoclonal antibody detecting CD4 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	5A8
Clonality	Monoclonal
Target	T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, CD4
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01730
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	T-cell surface glycoprotein CD4 is a ~51 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD4 Antibody (5A8).
Western blot

Western blot analysis was performed using anti-CD4 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD4 (Catalog No: HF998011)
Lane 2: negative control
SDS-PAGE

SDS-PAGE for Anti-Human CD4 Antibody (5A8)

References

Datasheet

Anti-Human CD4 Recombinant Antibody (5A8).pdf

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Formula

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Anti-Human CD4 Recombinant Antibody (5A8)