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Anti-Human CD4 Recombinant Antibody (OKT4A), APC (HF998337)

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Overview
Catalog No.HF998337
Description
Anti-Human CD4 Antibody (OKT4A), APC [OKT4A] (HF998337) is a mouse monoclonal antibody detecting CD4 in FCM, IF. Suitable for Human.
Highlights
  • Flow Cytometry — Validated for flow cytometry applications.
  • Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivityHuman
ApplicationsFCM, IF
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDOKT4A
ConjugationAPC
Clonality Monoclonal
Target T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, CD4
Endotoxin level Please contact with the lab for this information.
Accession P01730
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

T-cell surface glycoprotein CD4 is a ~51 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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