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Anti-Human CD49a/ITGA1 Recombinant Antibody (AQC2) (HX227107)

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Anti-Human CD49a/ITGA1 Recombinant Antibody (AQC2)

Anti-Human CD49a/ITGA1 Recombinant Antibody (AQC2)

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Overview

Catalog No.	HX227107
Description	Anti-Human CD49a/ITGA1 Antibody (AQC2) [AQC2] (HX227107) is a mouse monoclonal antibody detecting ITGA1 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	AQC2
Clonality	Monoclonal
Target	ITGA1, CD49a, CD49 antigen-like family member A, Laminin and collagen receptor, Integrin alpha-1, VLA-1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P56199
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Integrin alpha-1 (CD49a/ITGA1) is a ~130 kDa protein. Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CD49a antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD49a antibody monoclonal antibody (Catalog # HX227107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD49a/ITGA1 Recombinant Antibody (AQC2).pdf

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Anti-Human CD49a/ITGA1 Recombinant Antibody (AQC2)