Anti-Human CD5 Polyclonal Antibody (HY046014)

Name: Anti-Human CD5 Polyclonal Antibody
Brand: abinScience
SKU: HY046014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY046014

Description

Anti-Human CD5 Polyclonal Antibody (HY046014) is a rabbit polyclonal antibody detecting CD5 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD5 (Arg158-Gln368).

Target

T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD5, CD5, LEU1

Purification

Purified by antigen affinity column.

Accession

P06127

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

T-cell surface glycoprotein CD5 is a ~54 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation.

1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049

2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158

3. Roa, NS. et al. (2013) Biochemical and biophysical research communications 432, 52-9. PMID: 23376399

4. Garaud, S. et al. (2018) Cellular & molecular immunology 15, 158-170. PMID: 27499044

Note

For research use only.

Images

References

Datasheet