Anti-Human CD59 Polyclonal Antibody (HB615034)

Name: Anti-Human CD59 Polyclonal Antibody
Brand: abinScience
SKU: HB615034
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB615034

Description

Anti-Human CD59 Polyclonal Antibody (HB615034) is a rabbit polyclonal antibody detecting CD59 in ELISA, IHC, WB. Suitable for Pongo abelii and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human CD59 (Ser25-Pro128).

Target

CD59

Purification

Purified by antigen affinity column.

Accession

P13987

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-inhibitory protein, MAC-IP, MEM43 antigen, Membrane attack complex inhibition factor, MACIF, Membrane inhibitor of reactive lysis, MIRL, Protectin, CD59, CD59, MIC11, MIN1, MIN2, MIN3, MSK21

Background

CD59 glycoprotein is a ~14 kDa protein. Potent inhibitor of the complement membrane attack complex (MAC) action, which protects human cells from damage during complement activation. Acts by binding to the beta-haipins of C8 (C8A and C8B) components of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore.

1. Farkas, I. et al. (2002) The Journal of physiology 539, 537-45. PMID: 11882685

2. Meri, S. et al. (1990) Immunology 71, 1-9. PMID: 1698710

3. Okada, H. et al. (1989) Biochemical and biophysical research communications 162, 1553-9. PMID: 2475111

4. Davies, A. et al. (1989) The Journal of experimental medicine 170, 637-54. PMID: 2475570

5. Sugita, Y. et al. (1989) Journal of biochemistry 106, 555-7. PMID: 2606909

6. Bodian, DL. et al. (1997) The Journal of experimental medicine 185, 507-16. PMID: 9053451

7. Couves, EC. et al. (2023) Nature communications 14, 890. PMID: 36797260

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD59 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MDA-MB-231 cell lysate
Lane 2: BxPC-3 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: HUVEC cell lysate

References

Datasheet

Anti-Human CD59 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CD59 Polyclonal Antibody (HB615034)

Overview

Images

References

Datasheet

SDS