Anti-Human CEACAM-5/CD66e Recombinant Antibody (MN14) (HY546207)

Name: Anti-Human CEACAM-5/CD66e Recombinant Antibody (MN14)
Brand: abinScience
SKU: HY546207
Price: 196 USD
Availability: InStock

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Overview

Catalog No.	HY546207
Description	Anti-Human CEACAM-5/CD66e Recombinant Antibody (MN14) (HY546207) is a human monoclonal antibody detecting CEACAM-5/CD66e in FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivity	Human
Applications	FCM
Host species	Human
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone ID	MN14
Tested applications	FCM
Target	CD66e, CEA, CEA cell adhesion molecule 5, CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Cell adhesion molecule CEACAM5, Meconium antigen 100
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P06731
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Cell adhesion molecule CEACAM5 (CD66e) is a ~76 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells. 1. Taheri, M. et al. (2000) The Journal of biological chemistry 275, 26935-43. PMID: 10864933 2. Ordoñez, C. et al. (2000) Cancer research 60, 3419-24. PMID: 10910050 3. Oikawa, S. et al. (1989) Biochemical and biophysical research communications 164, 39-45. PMID: 2803308
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD66e/CEA/CEACAM5 Antibody (MN14).
Flow Cytometry

Flow-cytometry using anti-human CD66e antibody.CD66e Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD66e antibody monoclonal antibody (Catalog # HY546207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD66e antibody.BxPC3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD66e antibody monoclonal antibody (Catalog # HY546207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD66e monoclonal antibody (Catalog HY546207, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CEACAM-5/CD66e Recombinant Antibody (MN14).pdf

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Anti-Human CEACAM-5/CD66e Recombinant Antibody (MN14) (HY546207)

Overview

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References

Datasheet

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