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Anti-Human CEACAM-5/CD66e Recombinant Antibody (SAA0030), FITC (HY546117)

Anti-Human CEACAM-5/CD66e Recombinant Antibody (SAA0030), FITC
Anti-Human CEACAM-5/CD66e Recombinant Antibody (SAA0030), FITC
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Overview
Catalog No.HY546117
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA0030
ConjugationFITC
Clonality Monoclonal
Target Meconium antigen 100, CEACAM5, CEA, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen
Endotoxin level Please contact with the lab for this information.
Associated products Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), FITC
Accession P06731
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Cell adhesion molecule CEACAM5 (CD66e) is a ~76 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.

1. Taheri, M. et al. (2000) The Journal of biological chemistry 275, 26935-43. PMID: 10864933
2. Ordoñez, C. et al. (2000) Cancer research 60, 3419-24. PMID: 10910050
3. Oikawa, S. et al. (1989) Biochemical and biophysical research communications 164, 39-45. PMID: 2803308
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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  • Anti-Human CEACAM-5/CD66e Recombinant Antibody (SAA0030), FITC

    Flow cytometry

    Flow-cytometry using FITC anti-human CD66e antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD66e monoclonal antibody (Catalog HY546117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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