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Anti-Human CD69 Recombinant Antibody (SAA0819), PerCP (HC273247)

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Overview

Catalog No.	HC273247
Description	Anti-Human CD69 Antibody (SAA0819), PerCP [SAA0819] (HC273247) is a rabbit monoclonal antibody detecting CD69 in FCM. Suitable for Human. Highlights ●Rabbit Monoclonal — High specificity, affinity, and excellent lot-to-lot consistency. ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	FCM
Host species	Rabbit
Isotype	IgG
Clone ID	SAA0819
Conjugation	PerCP
Clonality	Monoclonal
Target	AIM, CD69, C-type lectin domain family 2 member C, Early T-cell activation antigen p60, BL-AC/P26, GP32/28, MLR-3, Early activation antigen CD69, Leukocyte surface antigen Leu-23, CLEC2C, EA1, Activation inducer molecule
Endotoxin level	Please contact with the lab for this information.
Accession	Q07108
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Early activation antigen CD69 is a ~22 kDa protein. Transmembrane protein expressed mainly on T-cells resident in mucosa that plays an essential role in immune cell homeostasis. Rapidly expressed on the surface of platelets, T-lymphocytes and NK cells upon activation by various stimuli, such as antigen recognition or cytokine signaling, stimulates different signaling pathways in different cell types. Negatively regulates Th17 cell differentiation through its carbohydrate dependent interaction with galectin-1/LGALS1 present on immature dendritic cells. Association of CD69 cytoplasmic tail with the JAK3/STAT5 signaling pathway regulates the transcription of RORgamma/RORC and, consequently, differentiation toward the Th17 lineage. Also acts via the S100A8/S100A9 complex present on peripheral blood mononuclear cells to promote the conversion of naive CD4 T-cells into regulatory T-cells. 1. de la Fuente, H. et al. (2014) Molecular and cellular biology 34, 2479-87. PMID: 24752896 2. Lin, CR. et al. (2015) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 29, 5006-17. PMID: 26296369 3. Jiménez-Fernández, M. et al. (2022) Cellular and molecular life sciences : CMLS 79, 468. PMID: 35930205 4. Jiang, Y. et al. (2021) Clinical and translational medicine 11, e563. PMID: 34841721 5. Chen, H. et al. (2023) eLife 12. PMID: 37039481
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Anti-Human CD69 Recombinant Antibody (SAA0819), PerCP.pdf

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Anti-Human CD69 Recombinant Antibody (SAA0819), PerCP