Anti-Human CD70 Recombinant Antibody (1F6), APC (HW717237)

Name: Anti-Human CD70 Recombinant Antibody (1F6), APC
Brand: abinScience
SKU: HW717237
Price: 256 USD
Availability: InStock

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Overview

Catalog No.	HW717237
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	1F6
Conjugation	APC
Clonality	Monoclonal
Target	CD27 ligand, CD70, TNFSF7, CD27LG, CD27L, CD70 antigen, Tumor necrosis factor ligand superfamily member 7, CD27-L
Endotoxin level	Please contact with the lab for this information.
Associated products	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
Accession	P32970
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	CD70 antigen is a ~21 kDa protein. Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells. 1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863 2. Abolhassani, H. et al. (2017) The Journal of experimental medicine 214, 91-106. PMID: 28011864 3. Goodwin, RG. et al. (1993) Cell 73, 447-56. PMID: 8387892
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow cytometry

Flow-cytometry using APC anti-human CD70 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD70 monoclonal antibody (Catalog HW717237, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet