Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6) (HW717207)

Name: Anti-Human CD27 Ligand/TNFSF7/CD70 Recombinant Antibody (1F6)
Brand: abinScience
SKU: HW717207
Price: 196 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HW717207
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	1F6
Clonality	Monoclonal
Target	CD27 ligand, CD70, TNFSF7, CD27LG, CD27L, CD70 antigen, Tumor necrosis factor ligand superfamily member 7, CD27-L
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P32970
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	CD70 antigen is a ~21 kDa protein. Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells. The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells. 1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863 2. Abolhassani, H. et al. (2017) The Journal of experimental medicine 214, 91-106. PMID: 28011864 3. Goodwin, RG. et al. (1993) Cell 73, 447-56. PMID: 8387892
Note	For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Human CD70 Antibody (1F6).
Flow CytoMetry

Flow-cytometry using anti-human CD70 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD70 monoclonal antibody (Catalog: HW717207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.