Anti-Human Transferrin R/TfR Recombinant Antibody (JR-141), FITC (HY036117)

Name: Anti-Human Transferrin R/TfR Recombinant Antibody (JR-141), FITC
Brand: abinScience
SKU: HY036117
Price: 196 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.	HY036117
Species reactivity	Human
Applications	ELISA, FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	JR-141
Conjugation	FITC
Clonality	Monoclonal
Tested applications	FCM
Target	Transferrin receptor protein 1, TfR1, T9, Trfr, p90, CD71, TFRC, TfR, sTfR, TR
Endotoxin level	Please contact with the lab for this information.
Associated products	Human IgG1 Recombinant Isotype Control Antibody (HyHEL-10), FITC
Accession	P02786
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Transferrin receptor protein 1 (CD71/TFRC) is a ~84 kDa protein. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. 1. Senyilmaz, D. et al. (2015) Nature 525, 124-8. PMID: 26214738 2. Jabara, HH. et al. (2016) Nature genetics 48, 74-8. PMID: 26642240
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using FITC anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD71 monoclonal antibody (Catalog: HY036117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet