Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0033) (HB209207)

Name: Anti-Human 5'-Nucleotidase/CD73 Recombinant Antibody (SAA0033)
Brand: abinScience
SKU: HB209207
Price: 196 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.	HB209207
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1, Kappa
Clone ID	SAA0033
Clonality	Monoclonal
Target	CD73, 5'-nucleotidase, NT5E, NT5, 5'-NT, Ecto-5'-nucleotidase, NTE
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P21589
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	5'-nucleotidase (CD73/NT5E) is a ~63 kDa protein. Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate. Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms. Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN. 1. Garavaglia, S. et al. (2012) The Biochemical journal 441, 131-41. PMID: 21933152 2. Heuts, DP. et al. (2012) Chembiochem : a European journal of chemical biology 13, 2384-91. PMID: 22997138 3. Knapp, K. et al. (2012) Structure (London, England : 1993) 20, 2161-73. PMID: 23142347 4. Fausther, M. et al. (2014) PloS one 9, e98568. PMID: 24887587 5. Scaletti, E. et al. (2021) Purinergic signalling 17, 693-704. PMID: 34403084
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CD73 antibody. Human U-87 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD73 antibody monoclonal antibody (Catalog # HB209207 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet