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Anti-Human LILRB3/CD85a/ILT5 Recombinant Antibody (A1) (HF547107)

Anti-Human LILRB3/CD85a/ILT5 Recombinant Antibody (A1)
Anti-Human LILRB3/CD85a/ILT5 Recombinant Antibody (A1)
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Overview
Catalog No.HF547107
Species reactivityHuman
ApplicationsELISA, FCM
Host speciesHuman
IsotypeIgG1, lambda
Clone IDA1
Clonality Monoclonal
Target CD85a, ILT-5, Monocyte inhibitory receptor HL9, LILRB3, Immunoglobulin-like transcript 5, CD85 antigen-like family member A, ILT5, Leukocyte immunoglobulin-like receptor 3, LIR-3, Leukocyte immunoglobulin-like receptor subfamily B member 3, LIR3
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession O75022
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Leukocyte immunoglobulin-like receptor subfamily B member 3 (CD85a/LILRB3) is a ~69 kDa protein. May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM).

Note For research use only.
Images
  • Anti-Human LILRB3/CD85a/ILT5 Recombinant Antibody (A1)

    Flow-cytometry

    Flow-cytometry using anti-human CD85a antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD85a antibody monoclonal antibody (Catalog # HF547107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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