Anti-Human LILRB2/CD85d/ILT4 Recombinant Antibody (J19.H1) (HV255107)

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Overview

Catalog No.	HV255107
Description	Anti-Human CD85d/LILRB2 Antibody (J19.H1) [J19.H1] (HV255107) is a mouse monoclonal antibody detecting LILRB2 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	J19.H1
Clonality	Monoclonal
Target	CD85 antigen-like family member D, Monocyte/macrophage immunoglobulin-like receptor 10, MIR-10, ILT4, LIR-2, ILT-4, Immunoglobulin-like transcript 4, MIR10, CD85d, Leukocyte immunoglobulin-like receptor subfamily B member 2, Leukocyte immunoglobulin-like receptor 2, LIR2, LILRB2
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q8N423
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Leukocyte immunoglobulin-like receptor subfamily B member 2 (CD85d/LILRB2) is a ~65 kDa protein. Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance. Competes with CD8A for binding to class I MHC antigens. 1. Lepin, EJ. et al. (2000) European journal of immunology 30, 3552-61. PMID: 11169396 2. Shiroishi, M. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 8856-61. PMID: 12853576 3. Shiroishi, M. et al. (2006) The Journal of biological chemistry 281, 10439-47. PMID: 16455647 4. Gregori, S. et al. (2010) Blood 116, 935-44. PMID: 20448110 5. Köstlin, N. et al. (2017) European journal of immunology 47, 374-384. PMID: 27859042 6. Chang, CC. et al. (2002) Nature immunology 3, 237-43. PMID: 11875462 8. Fanger, NA. et al. (1998) European journal of immunology 28, 3423-34. PMID: 9842885
Note	For research use only.

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Bioactivity

Detects CD85d/LILRB2/ILT4 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD85d antibody.CD85d Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD85d antibody monoclonal antibody (Catalog # HV255107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD85d antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD85d antibody monoclonal antibody (Catalog # HV255107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.