Anti-Human Complement Component C5aR1 Polyclonal Antibody (HB064014)

Name: Anti-Human Complement Component C5aR1 Polyclonal Antibody
Brand: abinScience
SKU: HB064014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB064014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD88/C5AR1 (Arg175-Arg206).

Target

CD88, C5AR1, C5R1, C5AR, C5a-R, C5aR, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor

Purification

Purified by antigen affinity column.

Accession

P21730

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

C5a anaphylatoxin chemotactic receptor 1 (CD88/C5AR1) is a ~39 kDa protein. Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, stimulating chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. C5AR1 is coupled to G(i)/G(o) (GNAI1 or GNAO1) G alpha proteins and mediates inhibition of adenylate cyclase. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events.

1. Christophe, T. et al. (2000) The Journal of biological chemistry 275, 1656-64. PMID: 10636859

2. Postma, B. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 172, 6994-7001. PMID: 15153520

3. Gerard, NP. et al. (1991) Nature 349, 614-7. PMID: 1847994

4. Robertson, N. et al. (2018) Nature 553, 111-114. PMID: 29300009

5. Feng, Y. et al. (2023) Cell research 33, 312-324. PMID: 36806352

6. Monk, PN. et al. (1995) The Journal of biological chemistry 270, 16625-9. PMID: 7622471

7. DeMartino, JA. et al. (1994) The Journal of biological chemistry 269, 14446-50. PMID: 8182049

8. Chen, Z. et al. (1998) The Journal of biological chemistry 273, 10411-9. PMID: 9553099

9. Yadav, MK. et al. (2023) Cell 186, 4956-4973.e21. PMID: 37852260

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-C5AR1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: U937 cell lysate

References

Datasheet

Anti-Human Complement Component C5aR1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human Complement Component C5aR1 Polyclonal Antibody (HB064014)

Overview

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References

Datasheet

SDS

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