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Anti-Human CEL Polyclonal Antibody (HB688014)

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Overview
Catalog No.HB688014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CEL (Asp117-Glu361).
Target Carboxyl ester lipase, Bucelipase, Sterol esterase, Bile salt-activated lipase, BAL, Bile salt-stimulated lipase, Cholesterol esterase, Pancreatic lysophospholipase, CEL, BSSL
Purification Purified by antigen affinity column.
Accession P19835
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Bile salt-activated lipase (CEL) is a ~79 kDa protein. Catalyzes the hydrolysis of a wide range of substrates including cholesteryl esters, phospholipids, lysophospholipids, di- and tri-acylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs). Preferentially hydrolyzes FAHFAs with the ester bond further away from the carboxylate. Unsaturated FAHFAs are hydrolyzed more quickly than saturated FAHFAs. Has an essential role in the complete digestion of dietary lipids and their intestinal absorption, along with the absorption of fat-soluble vitamins.

1. Tanaka, H. et al. (1999) Journal of biochemistry 125, 883-90. PMID: 10220579
2. Kolar, MJ. et al. (2016) Biochemistry 55, 4636-41. PMID: 27509211
3. Xiao, X. et al. (2016) The Journal of biological chemistry 291, 23224-23236. PMID: 27650499
4. Hui, DY. et al. (1993) The Biochemical journal 291 ( Pt 1), 65-9. PMID: 8471055
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CEL Polyclonal Antibody

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