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Anti-Human CENPB Polyclonal Antibody (HY318014)

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Anti-Human CENPB Polyclonal Antibody
Anti-Human CENPB Polyclonal Antibody
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Overview
Catalog No.HY318014
Description
Anti-Human CENPB Polyclonal Antibody (HY318014) is a rabbit polyclonal antibody detecting CENPB in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CENPB (Ser181-Gly392).
Target Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB
Purification Purified by antigen affinity column.
Accession P07199
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Major centromere autoantigen B (CENPB) is a ~65 kDa protein. Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable).

1. Tanaka, Y. et al. (2001) The EMBO journal 20, 6612-8. PMID: 11726497
Note For research use only.
Images
  • Anti-Human CENPB Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CENPB polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: PC-12 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: Daudi cell lysate
    Lane 7: A549 cell lysate
    Lane 8: A431 cell lysate
    Lane 9: PC-3 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CENPB Polyclonal Antibody

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