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Anti-Human CEP250 Polyclonal Antibody (HD660014)

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Overview
Catalog No.HD660014
Description
Anti-Human CEP250 Polyclonal Antibody (HD660014) is a rabbit polyclonal antibody detecting CEP250. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CEP250 (Arg40-Thr293).
Target 250 kDa centrosomal protein, C-Nap1, CEP2, CEP250, CNAP1, Centrosomal Nek2-associated protein 1, Centrosomal protein 2, Centrosome-associated protein CEP250, Cep250
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9BV73
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Centrosome-associated protein CEP250 is a ~281 kDa protein. Plays an important role in centrosome cohesion during interphase. Recruits CCDC102B to the proximal ends of centrioles. Maintains centrosome cohesion by forming intercentriolar linkages. Accumulates at the proximal end of each centriole, forming supramolecular assemblies with viscous material properties that promote organelle cohesion. May be involved in ciliogenesis.

1. Xia, Y. et al. (2018) Journal of cell science 131. PMID: 30404835
2. Mahen, R. (2022) PLoS biology 20, e3001854. PMID: 36282799
3. de Castro-Miró, M. et al. (2016) PloS one 11, e0168966. PMID: 28005958
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human CEP250 Polyclonal Antibody

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