Anti-Human CFD Polyclonal Antibody (HF697014)

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Overview

Catalog No.

HF697014

Description

Anti-Human CFD Polyclonal Antibody (HF697014) is a rabbit polyclonal antibody detecting CFD. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CFD (Met1-Ala253).

Target

Adipsin, C3 convertase activator, CFD, Complement factor D, DF, EC:3.4.21.46, PFD, Properdin factor D

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P00746

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Complement factor D (CFD) is a ~27 kDa protein. Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3. The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases. Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway.

1. Forneris, F. et al. (2010) Science (New York, N.Y.) 330, 1816-20. PMID: 21205667

2. Katschke, KJ. et al. (2012) The Journal of biological chemistry 287, 12886-92. PMID: 22362762

3. Niemann, MA. et al. (1980) Biochemistry 19, 1576-83. PMID: 6769474

4. Volanakis, JE. et al. (1977) Journal of immunology (Baltimore, Md. : 1950) 119, 337-42. PMID: 874324

5. Hourcade, DE. et al. (1998) The Journal of biological chemistry 273, 25996-6000. PMID: 9748277

Note

For research use only

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References

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