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Human CFD Recombinant Protein (N-His) (HF697012)

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Human CFD Recombinant Protein (N-His)

Human CFD Recombinant Protein (N-His)

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Overview

Catalog No.	HF697012
Description	Recombinant Human CFD Protein, N-His (HF697012) expressed in E. coli, spanning Met1-Ala253. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P00746
Protein length	Met1-Ala253
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PFD, Adipsin, C3 convertase activator, Complement factor D, CFD, DF, Properdin factor D
Background	Complement factor D (CFD) is a ~27 kDa protein. Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3. The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases. Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway. 1. Forneris, F. et al. (2010) Science (New York, N.Y.) 330, 1816-20. PMID: 21205667 2. Katschke, KJ. et al. (2012) The Journal of biological chemistry 287, 12886-92. PMID: 22362762 3. Niemann, MA. et al. (1980) Biochemistry 19, 1576-83. PMID: 6769474 4. Volanakis, JE. et al. (1977) Journal of immunology (Baltimore, Md. : 1950) 119, 337-42. PMID: 874324 5. Hourcade, DE. et al. (1998) The Journal of biological chemistry 273, 25996-6000. PMID: 9748277
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human CFD Protein, N-His

References

HF697012-SDS-PAGE-1.jpg

Datasheet

Human CFD Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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Human CFD Recombinant Protein (N-His)