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Anti-Human CHCHD3 Polyclonal Antibody (HP686014)

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Anti-Human CHCHD3 Polyclonal Antibody
Anti-Human CHCHD3 Polyclonal Antibody
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Overview
Catalog No.HP686014
Description
Anti-Human CHCHD3 Polyclonal Antibody (HP686014) is a rabbit polyclonal antibody detecting CHCHD3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CHCHD3 (Ser58-Gly227).
Target MICOS complex subunit MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, MINOS3, MIC19, CHCHD3
Purification Purified by antigen affinity column.
Accession Q9NX63
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

MICOS complex subunit MIC19 (CHCHD3) is a ~26 kDa protein. Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription.

1. Ott, C. et al. (2015) PloS one 10, e0120213. PMID: 25781180
2. Stephan, T. et al. (2020) The EMBO journal 39, e104105. PMID: 32567732
3. Hu, C. et al. (2020) Cell death & disease 11, 940. PMID: 33130824
4. Liu, H. et al. (2012) PloS one 7, e34832. PMID: 22567091
Note For research use only.
Images
  • Anti-Human CHCHD3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CHCHD3 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Hepg2 cell lysate
    Lane 5: A549 cell lysate
    Lane 6: 293T cell lysate
    Lane 7: Mouse kidney lysate
    Lane 8: Mouse liver lysate
    Lane 9: Mouse heart lysate
    Lane 10: Rat kidney lysate
    Lane11: Rat Liver lysate
    Lane12: Rat heart lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CHCHD3 Polyclonal Antibody

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