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Anti-Human CHD1 Polyclonal Antibody (HT401014)

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Overview
Catalog No.HT401014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CHD1 (Ser1127-Thr1285).
Target ATP-dependent helicase CHD1, CHD1, Chromodomain-helicase-DNA-binding protein 1, CHD-1
Purification Purified by antigen affinity column.
Accession O14646
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

ATP-dependent chromatin remodeler CHD1 is a ~196 kDa protein. ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome.

1. Sims, RJ. et al. (2007) Molecular cell 28, 665-76. PMID: 18042460
2. Pilarowski, GO. et al. (2018) Journal of medical genetics 55, 561-566. PMID: 28866611
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human CHD1 Polyclonal Antibody

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