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Anti-Human CHD3 IP Recombinant Antibody (SAA0258) (HC478013)

Anti-Human CHD3 IP Recombinant Antibody (SAA0258)
Anti-Human CHD3 IP Recombinant Antibody (SAA0258)
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Overview
Catalog No.HC478013
Species reactivityHuman
ApplicationsELISA, IF, IP
Host speciesMouse
IsotypeIgG2a
Clone IDSAA0258
Clonality Monoclonal
Target CHD-3, Mi2-alpha, ATP-dependent helicase CHD3, Mi-2 autoantigen 240 kDa protein, Chromodomain-helicase-DNA-binding protein 3, CHD3, hZFH, Zinc finger helicase
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q12873
RRID Anti-Human CHD3 IP Antibody (SAA0258) (abinScience Cat# HC478013, RRID:AB_3729792)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

ATP-dependent chromatin remodeler CHD3 is a ~226 kDa protein. ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Involved in transcriptional repression as part of the NuRD complex. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

1. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666
2. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440
3. Snijders Blok, L. et al. (2018) Nature communications 9, 4619. PMID: 30397230
4. Tong, JK. et al. (1998) Nature 395, 917-21. PMID: 9804427
5. Yamashita, D. et al. (2016) The Journal of biological chemistry 291, 11619-34. PMID: 27068747
6. Sillibourne, JE. et al. (2007) Molecular biology of the cell 18, 3667-80. PMID: 17626165
Note For research use only.
Images
  • Anti-Human CHD3 IP Recombinant Antibody (SAA0258)

    SDS-PAGE

    SDS-PAGE for Anti-Human CHD3 IP Antibody (SAA0258)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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