

| Catalog No. | HC478013 | ||||
|---|---|---|---|---|---|
| Species reactivity | Human | ||||
| Applications | ELISA, IF, IP | ||||
| Host species | Mouse | ||||
| Isotype | IgG2a | ||||
| Clone ID | SAA0258 | ||||
| Clonality | Monoclonal | ||||
| Target | CHD-3, Mi2-alpha, ATP-dependent helicase CHD3, Mi-2 autoantigen 240 kDa protein, Chromodomain-helicase-DNA-binding protein 3, CHD3, hZFH, Zinc finger helicase | ||||
| Endotoxin level | Please contact with the lab for this information. | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Accession | Q12873 | ||||
| RRID | Anti-Human CHD3 IP Antibody (SAA0258) (abinScience Cat# HC478013, RRID:AB_3729792) |
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| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
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| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Background | ATP-dependent chromatin remodeler CHD3 is a ~226 kDa protein. ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Involved in transcriptional repression as part of the NuRD complex. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. 1. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666 2. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440 3. Snijders Blok, L. et al. (2018) Nature communications 9, 4619. PMID: 30397230 4. Tong, JK. et al. (1998) Nature 395, 917-21. PMID: 9804427 5. Yamashita, D. et al. (2016) The Journal of biological chemistry 291, 11619-34. PMID: 27068747 6. Sillibourne, JE. et al. (2007) Molecular biology of the cell 18, 3667-80. PMID: 17626165 | ||||
| Note | For research use only. |

SDS-PAGE for Anti-Human CHD3 IP Antibody (SAA0258)
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