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Anti-Human CHKA Polyclonal Antibody (HW469014)

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Anti-Human CHKA Polyclonal Antibody
Anti-Human CHKA Polyclonal Antibody
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Overview
Catalog No.HW469014
Description
Anti-Human CHKA Polyclonal Antibody (HW469014) is a rabbit polyclonal antibody detecting CHKA in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CHKA (Asp138-Leu376).
Target Choline kinase alpha, CK, 2.7.1.32, CHETK-alpha, Ethanolamine kinase, EK, 2.7.1.82, CHKA, CHK, CKI
Purification Purified by antigen affinity column.
Accession P35790
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Choline kinase alpha (CHKA) is a ~52 kDa protein. Plays a key role in phospholipid biosynthesis by catalyzing the phosphorylation of free choline to phosphocholine, the first step in phosphatidylcholine biosynthesis. Also phosphorylates ethanolamine, thereby contributing to phosphatidylethanolamine biosynthesis. Has higher activity with choline. May contribute to tumor cell growth.

1. Malito, E. et al. (2006) Journal of molecular biology 364, 136-51. PMID: 17007874
2. Gallego-Ortega, D. et al. (2009) PloS one 4, e7819. PMID: 19915674
3. Hudson, CS. et al. (2013) Biochimica et biophysica acta 1834, 1107-16. PMID: 23416529
4. Liu, R. et al. (2021) Molecular cell 81, 2722-2735.e9. PMID: 34077757
Note For research use only.
Images
  • Anti-Human CHKA Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CHKA polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: Raji cell lysate
    Lane 3: Mouse testis lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CHKA Polyclonal Antibody

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