Anti-Human CLEC5A/MDL1 Recombinant Antibody (DX246) (HP329107)

Name: Anti-Human CLEC5A/MDL1 Recombinant Antibody (DX246)
Brand: abinScience
SKU: HP329107
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HP329107
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	DX246
Clonality	Monoclonal
Target	C-type lectin superfamily member 5, C-type lectin domain family 5 member A, CLEC5A, MDL-1, CLECSF5, MDL1, Myeloid DAP12-associating lectin 1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9NY25
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	C-type lectin domain family 5 member A (CLEC5A/MDL1) is a ~21 kDa protein. Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. 1. Bakker, AB. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 9792-6. PMID: 10449773
Note	For research use only.

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SEC-HPLC

SEC-HPLC detection for Anti-Human CLEC5A/MDL1 Antibody (DX246).
Bioactivity

Detects CLEC5A/MDL1 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CLEC5A antibody.CLEC5A Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CLEC5A antibody monoclonal antibody (Catalog # HP329107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CLEC5A/MDL1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CLEC5A/MDL1 (Catalog No: HP329011)
Lane 2: negative control

References

Datasheet

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Overview