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Anti-Human CLPP Polyclonal Antibody (HS880014)

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Overview
Catalog No.HS880014
Description
Anti-Human CLPP Polyclonal Antibody (HS880014) is a rabbit polyclonal antibody detecting CLPP in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CLPP (Gly21-His247).
Target Endopeptidase Clp, ATP-dependent Clp protease proteolytic subunit, mitochondrial, CLPP
Purification Purified by antigen affinity column.
Accession Q16740
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) is a ~30 kDa protein. Protease component of the ClpXP complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The ClpXP complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Cleaves PINK1 in the mitochondrion.

1. Kang, SG. et al. (2002) The Journal of biological chemistry 277, 21095-102. PMID: 11923310
2. Kang, SG. et al. (2004) Journal of structural biology 148, 338-52. PMID: 15522782
3. Greene, AW. et al. (2012) EMBO reports 13, 378-85. PMID: 22354088
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CLPP Polyclonal Antibody

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