Anti-Human CPSF6 Polyclonal Antibody (HS787014)

Name: Anti-Human CPSF6 Polyclonal Antibody
Brand: abinScience
SKU: HS787014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HS787014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human CPSF6 (Lys79-Ser181).

Target

Pre-mRNA cleavage factor Im 68 kDa subunit, CFIM68, Protein HPBRII-4/7, Cleavage and polyadenylation specificity factor subunit 6, CPSF6, CFIm68, CPSF 68 kDa subunit, Cleavage and polyadenylation specificity factor 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit

Purification

Purified by antigen affinity column.

Accession

Q16630

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cleavage and polyadenylation specificity factor subunit 6 (CPSF6) is a ~59 kDa protein. Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery.

1. Brown, KM. et al. (2003) Molecular cell 12, 1467-76. PMID: 14690600

2. Zhu, Y. et al. (2018) Molecular cell 69, 62-74.e4. PMID: 29276085

3. Rüegsegger, U. et al. (1996) The Journal of biological chemistry 271, 6107-13. PMID: 8626397

4. Rüegsegger, U. et al. (1998) Molecular cell 1, 243-53. PMID: 9659921

5. Gruber, AR. et al. (2012) RNA biology 9, 1405-12. PMID: 23187700

6. Kim, S. et al. (2010) Genes to cells : devoted to molecular & cellular mechanisms 15, 1003-13. PMID: 20695905

Note

For research use only.

Images

References

Datasheet

Anti-Human CPSF6 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human CPSF6 Polyclonal Antibody (HS787014)

Overview

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Datasheet

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