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Anti-Human CRAT Polyclonal Antibody (HD657014)

Anti-Human CRAT Polyclonal Antibody
Anti-Human CRAT Polyclonal Antibody
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Overview
Catalog No.HD657014
Description
Anti-Human CRAT Polyclonal Antibody (HD657014) is a rabbit polyclonal antibody detecting CRAT. Suitable for Human, Rat, and Mouse.
Highlights
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CRAT (His31-Glu297).
Target CAT, CAT1, CRAT, Carnitine O-acetyltransferase, Carnitine acetylase, Carnitine acetyltransferase, CrAT, EC:2.3.1.137, EC:2.3.1.7
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P43155
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Carnitine O-acetyltransferase (CRAT) is a ~70 kDa protein. Catalyzes the reversible transfer of acyl groups from carnitine to coenzyme A (CoA) and regulates the acyl-CoA/CoA ratio. Also plays a crucial role in the transport of fatty acids for beta-oxidation. Responsible for the synthesis of short- and branched-chain acylcarnitines. Active towards some branched-chain amino acid oxidation pathway (BCAAO) intermediates. Trans-2-enoyl-CoAs and 2-methylacyl-CoAs are poor substrates.

1. Govindasamy, L. et al. (2004) Journal of structural biology 146, 416-24. PMID: 15099582
2. Drecourt, A. et al. (2018) American journal of human genetics 102, 266-277. PMID: 29395073
3. Violante, S. et al. (2013) Biochimica et biophysica acta 1832, 773-9. PMID: 23485643
Note For research use only
Images
  • Anti-Human CRAT Polyclonal Antibody

    Western blot

    Lane 1: Rat heart lysate
    Lane 2: Rat skeletal muscle lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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