Anti-Human CUL4A Polyclonal Antibody (HC663014)

Name: Anti-Human CUL4A Polyclonal Antibody
Brand: abinScience
SKU: HC663014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC663014

Description

Anti-Human CUL4A Polyclonal Antibody (HC663014) is a rabbit polyclonal antibody detecting CUL4A in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CUL4A (Arg666-Ala759).

Target

CUL-4A, CUL4A, Cullin-4A

Purification

Purified by antigen affinity column.

Accession

Q13619

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cullin-4A (CUL4A) is a ~87 kDa protein. Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN.

1. Higa, LA. et al. (2003) Nature cell biology 5, 1008-15. PMID: 14578910

2. Wertz, IE. et al. (2004) Science (New York, N.Y.) 303, 1371-4. PMID: 14739464

3. Hu, J. et al. (2004) Nature cell biology 6, 1003-9. PMID: 15448697

4. Nag, A. et al. (2004) Cancer research 64, 8152-5. PMID: 15548678

5. Matsuda, N. et al. (2005) DNA repair 4, 537-45. PMID: 15811626

6. Wang, H. et al. (2006) Molecular cell 22, 383-94. PMID: 16678110

7. Higa, LA. et al. (2006) Nature cell biology 8, 1277-83. PMID: 17041588

8. Han, J. et al. (2013) Cell 155, 817-29. PMID: 24209620

9. Chen, SH. et al. (2018) The EMBO journal 37. PMID: 30166453

10. Maiani, E. et al. (2021) Nature 592, 799-803. PMID: 33854232

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CUL4A polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Western blot

Western blot analysis was performed using anti-CUL4A polyclonal antibody at 1μg/ml on various samples.
Lane 1: PC-12 cell lysate
Lane 2: Raji cell lysate

References

Datasheet

Anti-Human CUL4A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human CUL4A Polyclonal Antibody (HC663014)

Overview

Images

References

Datasheet

SDS

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