Anti-Human CUL4B Polyclonal Antibody (HC617014)

Name: Anti-Human CUL4B Polyclonal Antibody
Brand: abinScience
SKU: HC617014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HC617014

Description

Anti-Human CUL4B Polyclonal Antibody (HC617014) is a rabbit polyclonal antibody detecting CUL4B in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human CUL4B (Gly728-Ala913).

Target

Cullin-4B, CUL-4B, CUL4B, KIAA0695

Purification

Purified by antigen affinity column.

Accession

Q13620

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cullin-4B (CUL4B) is a ~103 kDa protein. Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication.

1. Higa, LA. et al. (2003) Nature cell biology 5, 1008-15. PMID: 14578910

2. Higa, LA. et al. (2006) Cell cycle (Georgetown, Tex.) 5, 71-7. PMID: 16322693

3. Wang, H. et al. (2006) Molecular cell 22, 383-94. PMID: 16678110

4. Guerrero-Santoro, J. et al. (2008) Cancer research 68, 5014-22. PMID: 18593899

5. Fischer, ES. et al. (2011) Cell 147, 1024-39. PMID: 22118460

6. Koren, I. et al. (2018) Cell 173, 1622-1635.e14. PMID: 29779948

7. Chen, SH. et al. (2018) The EMBO journal 37. PMID: 30166453

8. Maiani, E. et al. (2021) Nature 592, 799-803. PMID: 33854232

9. Chaikovsky, AC. et al. (2021) Nature 592, 794-798. PMID: 33854239

10. Sjögren, B. et al. (2015) PloS one 10, e0123581. PMID: 25970626

Note

For research use only.

Images

References

Datasheet

Anti-Human CUL4B Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human CUL4B Polyclonal Antibody (HC617014)

Overview

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References

Datasheet

SDS

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