Anti-Human CYP24A1 Polyclonal Antibody (HC495014)

Name: Anti-Human CYP24A1 Polyclonal Antibody
Brand: abinScience
SKU: HC495014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC495014

Description

Anti-Human CYP24A1 Polyclonal Antibody (HC495014) is a rabbit polyclonal antibody in WB, IHC, ELISA.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

WB, IHC, ELISA

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human CYP24A1 (Lys81-Arg514).

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

Q07973

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

125-dihydroxyvitamin D(3) 24-hydroxylase mitochondrial, 24-OHase, Vitamin D(3) 24-hydroxylase, EC:1.14.15.16, Cytochrome P450 24A1, Cytochrome P450-CC24, CYP24A1

Background

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (CYP24A1) is a ~58 kDa protein. A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product. Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol.

1. Sakaki, T. et al. (2000) European journal of biochemistry 267, 6158-65. PMID: 11012668

2. Sakaki, T. et al. (2005) Frontiers in bioscience : a journal and virtual library 10, 119-34. PMID: 15574355

3. Hamamoto, H. et al. (2006) Molecular pharmacology 70, 120-8. PMID: 16617161

4. Tieu, EW. et al. (2014) The FEBS journal 281, 3280-96. PMID: 24893882

5. Roizen, JD. et al. (2018) The Journal of clinical investigation 128, 1913-1918. PMID: 29461981

6. Beckman, MJ. et al. (1996) Biochemistry 35, 8465-72. PMID: 8679605

Note

For research use only.

Images

References

Datasheet

Anti-Human CYP24A1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CYP24A1 Polyclonal Antibody (HC495014)

Overview

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References

Datasheet

SDS

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