Anti-Human CYP2D6 Polyclonal Antibody (HY091014)

Name: Anti-Human CYP2D6 Polyclonal Antibody
Brand: abinScience
SKU: HY091014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY091014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CYP2D6 (Leu236-Gln472).

Target

Debrisoquine 4-hydroxylase, CYP2DL1, Cytochrome P450 2D6, Cytochrome P450-DB1, CYPIID6, Cholesterol 25-hydroxylase, CYP2D6

Purification

Purified by antigen affinity column.

Accession

P10635

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cytochrome P450 2D6 (CYP2D6) is a ~55 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of fatty acids, steroids and retinoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 20-hydroxyeicosatetraenoic acid ethanolamide (20-HETE-EA) and 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling. Catalyzes the hydroxylation of carbon-hydrogen bonds.

1. Snider, NT. et al. (2008) The Journal of pharmacology and experimental therapeutics 327, 538-45. PMID: 18698000

2. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576

3. Mesaros, C. et al. (2010) Rapid communications in mass spectrometry : RCM 24, 3237-47. PMID: 20972997

4. Sridar, C. et al. (2011) Drug metabolism and disposition: the biological fate of chemicals 39, 782-8. PMID: 21289075

5. Honda, A. et al. (2011) Journal of lipid research 52, 1509-16. PMID: 21576599

6. Chen, H. et al. (2000) Drug metabolism and disposition: the biological fate of chemicals 28, 315-22. PMID: 10681376

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human CYP2D6 polyclonal antibody at 1ug/mL on recombinant Human CYP2D6 (Catalog No. HY091012).

References

Datasheet

Anti-Human CYP2D6 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CYP2D6 Polyclonal Antibody (HY091014)

Overview

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References

Datasheet

SDS

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