Anti-Human CYP46A1 Polyclonal Antibody (HV358014)

Name: Anti-Human CYP46A1 Polyclonal Antibody
Brand: abinScience
SKU: HV358014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV358014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CYP46A1 (His45-Cys500).

Target

Cholesterol 24-hydroxylase, Cholesterol 24-monooxygenase, CH24H, CYP46A1, Cytochrome P450 46A1, CYP46, Cholesterol 24S-hydroxylase

Purification

Purified by antigen affinity column.

Accession

Q9Y6A2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cholesterol 24-hydroxylase (CYP46A1) is a ~56 kDa protein. P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation. By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning. Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis. Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain.

1. Lund, EG. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 7238-43. PMID: 10377398

2. Mast, N. et al. (2003) Biochemistry 42, 14284-92. PMID: 14640697

3. Mast, N. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 9546-51. PMID: 18621681

4. Goyal, S. et al. (2014) Journal of lipid research 55, 1933-43. PMID: 25017465

5. Bodin, K. et al. (2002) The Journal of biological chemistry 277, 31534-40. PMID: 12077124

6. Mast, N. et al. (2017) The Journal of biological chemistry 292, 4913-4924. PMID: 28190002

7. Mast, N. et al. (2010) The Journal of biological chemistry 285, 31783-95. PMID: 20667828

Note

For research use only.

Images

References

Datasheet

Anti-Human CYP46A1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human CYP46A1 Polyclonal Antibody (HV358014)

Overview

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References

Datasheet

SDS

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