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Anti-Human CYP4F22 Polyclonal Antibody (HD330014)

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Overview
Catalog No.HD330014
Description
Anti-Human CYP4F22 Polyclonal Antibody (HD330014) is a rabbit polyclonal antibody detecting CYP4F22. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CYP4F22 (Asn66-Ala531).
Target CYP4F22, Cytochrome P450 4F22, EC:1.14.14.177, Ultra-long-chain fatty acid omega-hydroxylase
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6NT55
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Ultra-long-chain fatty acid omega-hydroxylase (CYP4F22) is a ~61 kDa protein. A cytochrome P450 monooxygenase involved in epidermal ceramide biosynthesis. Hydroxylates the terminal carbon (omega-hydroxylation) of ultra-long-chain fatty acyls (C28-C36) prior to ceramide synthesis. Contributes to the synthesis of three classes of omega-hydroxy-ultra-long chain fatty acylceramides having sphingosine, 6-hydroxysphingosine and phytosphingosine bases, all major lipid components that underlie the permeability barrier of the stratum corneum. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

1. Ohno, Y. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 7707-12. PMID: 26056268
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human CYP4F22 Polyclonal Antibody

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