Anti-Human D2HGDH Polyclonal Antibody (HV254014)

Name: Anti-Human D2HGDH Polyclonal Antibody
Brand: abinScience
SKU: HV254014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV254014

Description

Anti-Human D2HGDH Polyclonal Antibody (HV254014) is a rabbit polyclonal antibody detecting D2HGDH in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human D2HGDH (Arg56-Asp232).

Target

D2HGDH

Purification

Purified by antigen affinity column.

Accession

Q8N465

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

D-2-hydroxyglutarate dehydrogenase, mitochondrial, 1.1.99.39, D2HGDH, D2HGD

Background

D-2-hydroxyglutarate dehydrogenase, mitochondrial (D2HGDH) is a ~56 kDa protein. Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate. Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC). Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC.

1. Achouri, Y. et al. (2004) The Biochemical journal 381, 35-42. PMID: 15070399

2. Struys, EA. et al. (2005) American journal of human genetics 76, 358-60. PMID: 15609246

3. Struys, EA. et al. (2005) Annals of neurology 58, 626-30. PMID: 16037974

4. Kranendijk, M. et al. (2010) Human mutation 31, 279-83. PMID: 20020533

5. Yang, J. et al. (2021) Cell discovery 7, 3. PMID: 33431826

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-D2HGDH polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate

References

Datasheet

Anti-Human D2HGDH Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human D2HGDH Polyclonal Antibody (HV254014)

Overview

Images

References

Datasheet

SDS

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