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Anti-Human DCK Polyclonal Antibody (HB117014)

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Overview
Catalog No.HB117014
Description
Anti-Human DCK Polyclonal Antibody (HB117014) is a rabbit polyclonal antibody detecting dCK in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human DCK (Met1-Leu260).
Target dCK, Deoxyguanosine kinase, Deoxycytidine kinase, Deoxyadenosine kinase, DCK
Purification Purified by antigen affinity column.
Accession P27707
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Deoxycytidine kinase (DCK) is a ~30 kDa protein. Phosphorylates the deoxyribonucleosides deoxycytidine, deoxyguanosine and deoxyadenosine. Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.

1. Sabini, E. et al. (2003) Nature structural biology 10, 513-9. PMID: 12808445
2. Sabini, E. et al. (2008) Journal of molecular biology 378, 607-21. PMID: 18377927
3. Hazra, S. et al. (2009) Biochemistry 48, 1256-63. PMID: 19159229
4. Chottiner, EG. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 1531-5. PMID: 1996353
5. Hazra, S. et al. (2010) Biochemistry 49, 6784-90. PMID: 20614893
6. Smal, C. et al. (2010) Archives of biochemistry and biophysics 502, 44-52. PMID: 20637175
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human DCK Polyclonal Antibody

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