Anti-Human DDX1 Polyclonal Antibody (HP260014)

Name: Anti-Human DDX1 Polyclonal Antibody
Brand: abinScience
SKU: HP260014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP260014

Description

Anti-Human DDX1 Polyclonal Antibody (HP260014) is a rabbit polyclonal antibody detecting DDX1 in ELISA, IHC, WB. Suitable for Mouse, Bovine, Chicken, Human, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DDX1 (Gln72-Leu283).

Target

DEAD box protein retinoblastoma, ATP-dependent RNA helicase DDX1, DEAD box protein 1, DBP-RB, DDX1

Purification

Purified by antigen affinity column.

Accession

Q92499

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ATP-dependent RNA helicase DDX1 is a ~82 kDa protein. Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation.

1. Popow, J. et al. (2014) Nature 511, 104-7. PMID: 24870230

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-DDX1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: PC3 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: H1299 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: K562 cell lysate
Lane 9: Rat brain lysate
Lane 10: Mouse kidney lysate

References

Datasheet

Anti-Human DDX1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human DDX1 Polyclonal Antibody (HP260014)

Overview

Images

References

Datasheet

SDS

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