Anti-Human DDX21 Polyclonal Antibody (HP105014)

Name: Anti-Human DDX21 Polyclonal Antibody
Brand: abinScience
SKU: HP105014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP105014

Description

Anti-Human DDX21 Polyclonal Antibody (HP105014) is a rabbit polyclonal antibody detecting DDX21 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human DDX21 (Ala187-Ile563).

Target

DEAD box protein 21, DDX21, Gu-alpha, Nucleolar RNA helicase II, RH II/Gu, Nucleolar RNA helicase Gu, Nucleolar RNA helicase 2

Purification

Purified by antigen affinity column.

Accession

Q9NR30

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleolar RNA helicase 2 (DDX21) is a ~87 kDa protein. RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs. In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes. In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes.

1. Calo, E. et al. (2015) Nature 518, 249-53. PMID: 25470060

2. Song, C. et al. (2017) Genes & development 31, 1370-1381. PMID: 28790157

3. Sloan, KE. et al. (2015) Nucleic acids research 43, 553-64. PMID: 25477391

4. Westermarck, J. et al. (2002) The EMBO journal 21, 451-60. PMID: 11823437

5. Zhang, Y. et al. (2014) Breast cancer research : BCR 16, 449. PMID: 25260534

6. Valdez, BC. et al. (1997) European journal of biochemistry 250, 800-7. PMID: 9461305

9. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-DDX21 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: K562 cell lysate

References

Datasheet

Anti-Human DDX21 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human DDX21 Polyclonal Antibody (HP105014)

Overview

Images

References

Datasheet

SDS

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